Sentence examples for brief binding from inspiring English sources

In brief, binding of U2AF to the polypyrimidine tract promotes formation of a 5'-2' lariat linkage between the splice donor site and the branch point nucleotide.

This brief binding event is an important step in the process that activates the enzyme.

In brief, binding of the ATP involving conserved Arg residues forming a 'basic clamp' brings NTD and CTD from one subunit closer together (a 'closure' stage later released upon ATP hydrolysis), driving a 'swinging' motion of the neighbouring subunit CTD with some residues moving tens of Å (Satyshur et al., 2007; Reindl et al., 2013).

In brief, the binding to TNFR of PGRN and its-derived Atsttrin have been confirmed and extended by the group that originally reported the interactions, and also by numerous independent groups.

In brief, nonspecific binding was blocked by incubating membranes for 1 h at 4°C with Odyssey Blocking Buffer diluted 1∶1 with PBS.

In brief, unspecific binding was blocked using 1% BSA, 5% normal serum, 0.1% Tween 20 or Triton-X-100 in PBS ( = blocking buffer) for 1 h.

In brief, Na+ binding to the Na1 site is coupled through movements of Met311 to the structural transitions in HP2.

In brief, high binding plates (NUNC, Roskilde, Denmark) were incubated with appropriate concentration of capture antibodies against IL-8 and MCP-1 overnight before incubating with 1% bovine serum albumin (BSA) in PBS for 2 h.

In brief, the binding of one of two ligands, Serrate or Delta (Jagged1/2 and Delta1/3/4 in vertebrates, respectively), to the N receptor triggers a proteolytic cleavage of N, releasing its intracellular domain (NICD) and allowing it to shuttle into the nucleus.

In brief, DNA protein binding reactions used cell extract (20 μg of protein) and digoxigenin-11-ddUTP (DIG -labeled DIG -labeled100 fmol) in a total volume of 10 μl containing 10 mM HEPES (pH 7.6), 1 mM EDNA, 10 mM (NH4)2SO4, 1 mM DTT, 1% (w/v) Tween 20, 1 μg probesI–dC) and 30 mM KCl.

In brief, TF-binding regions were identified by using the peak-finding algorithm built into the NimbleScan software (Roche NimbleGen).