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The LTD was induced by LFS delivered at 1 Hz for 15 min (900 pulses) or a brief bath application of group I mGluR agonist DHPG (Tocris Cookson Ltd., Bristol, UK) for 5 min. Whole-cell recordings were made from hippocampal CA1 pyramidal cells by using a patch clamp amplifier (Axopatch 200B, Axon Instruments) under infrared differential interference contrast optics.
Brief bath application of 50 µM NMDA for 3 minutes at 37°C was applied to CORT-treated hippocampal cultured neurones to elicit a chemically induced form of long-term depression [21] and in dispersed hippocampal cultures, exposure to NMDA evokes internalization of synaptic GluR1- and GluR2-containing AMPARs [17], [21], [22], [23].
In slices from 5-week-old SAL-treated rats, a brief bath application of DHPG (50 µM) for 5 min elicited a robust LTD (50 min after washout of DHPG Group AA, 54.4±4.2% of baseline, n = 7, p<0.05; Group B, 58.5±3.5% of baseline, n = 8, p<0.05) (Figures 5A and 5B).
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Don't be put off by this quick and easy procedure, for when these plump-from-the-sea sand dwellers are good and fresh, they want for nothing more than the briefest bath in the hot clam broth that accompanies their arrival for the purpose of removing any extraneous sand particles.
So if you find yourself plagued by quickly spoiling fruits, start giving them a brief hot bath before you spread them out or chill them.
It works by subjecting mature cells to a brief acid bath and then tweaking culture conditions.
A brief cold water bath finishes these moist, intensely chocolatey little cakes off perfectly.
DOI: http://dx.doi.org/10.7554/eLife.06195.006 To test whether Bk directly excites motoneurons, we recorded activity from 200 lumbar motoneurons in response to a brief (≤2 min) bath application of Bk (8 µM).
250 μl of Elution Buffer (50 mM Tris HCl pH 8.0, 10 mM EDTA, 1 % SDS) was added prior to incubation at 65 °C for 15 min in a water bath with brief vortexing every 2 min. The beads were centrifuged at 13,300 rpm for 1 min and the supernatant saved as this contains the ChIP material.
Commissioner D'Angelo announced that Goodyear would fly the two blimps above NYC every night with lighted signs on their sides to take brief showers instead of baths and to stop running hot water on dishes and would also distribute leaflets on water conservation as another volunteer measure.
Keep baths as brief as possible to avoid prolonged exposure to the water.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com