In brief, a solution of c RGDfK -PEG1-SH (15.6 mg, 19.0 μmol) in phosphate buffer (500 μL, 0.1 M, pH 6.0) was added to a solution of DOTA-Mal4 (5 mg, 3.2 μmol) in Mec RGDfK -PEG1-SHfer (0.1 M, pH 5.0) 1:1 (250 μL) and the pH of the mixture was adjusted to 7.4 by the addition of phosphate buffer (0.5 M, pH 7.4, approximately 100 μL).
In brief, a solution of 50 mL of H2SO4, 10 g of K2S2O8, and 10 g of P2O5 was heated to 80 °C.
In brief, a solution of POPC/POPG (80/20 molar ratio) dissolved in chloroform was mixed in a round bottom flask, after which the solvent was completely evaporated.
In brief, a solution of β-carotene was prepared by dissolving 2 mg of β-carotene in 10 mL of chloroform and 2 mL of this solution was pipette into a 100 mL round-bottom flask.
In brief, a solution of 0.05 mL of ΜPtMS in 5.0 mL of HMImCl was added to 5.0 mL of a solution that contained the (ZnS x(CuInS2 1−x/ZnS (alloyed core/shell) nanoparticles solubilized in toluene by the presence of lipophilic 1-mercapto- n-dodecyl ligands.
In brief, a solution of L-leucine methyl ester hydrochloride (545.1 mg, 3.0 mmol) and DIEA (1.15 ml, 6.6 mmol) in DMF (4.5 ml) was added dropwise under vigorous stirring to a solution of 1,5-difluoro-2,4-dinitrobenzene 1,5-difluoro-2,4-dinitrobenzene 1,5-difluoro-2,4-dinitrobenzene 1,5-difluoro-2,4-dinitrobenzene 612.0
The CEP derivative of 1,2-dipalmitoyl sn-glycero-3-phosphoethanolamine (CEP-DPPE) was prepared in a similar method as that described by Lu et al. In brief, to a solution of DPPE (45 mg, 0.065 mmol) in 500 μL of CHCl3 was subsequently added triethylamine (25 μL, 0.247 mmol) and a solution of DOHA-Fm (22 mg, 0.065 mmol) in 500 μL of CHCl3.
After a brief equilibration in a solution of 0.1 M Tris HCl, 0.1 M NaCl and 50 mM MgCl2, pH 7.5 (GB1) at room temperature, the sections were transferred to blocking solution, containing 0.1 M Tris HCl, 0.15 M NaCl and 0.5% blocking regent (PerkinElmer).
In brief, 100 μl of a solution of phage, either directly from the selection or from a population grown on C600F in LB, was extracted with phenol chloroform:0.1% SDS, chloroform extracted, ethanol precipitated, resuspended in 50 μl of water, and passed through a Centri-Sep column (Princeton Separations, Adelphia, NJ) to remove unincorporated small molecules.
In brief, cells were lysed in a solution of 5 mM Tris-HCl (pH 7.4) and 1 mM EDTA containing 0.5% (w/v) Triton X-100 for 20 min on ice.
In brief, a methanol solution of mPEG-PLA and Solutol HS15 with different molar ratios was evaporated under vacuum at 60°C to form a homogeneous film.
