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We assessed the consequences of Zfy2 expression for fertility in the context of a single X chromosome from breeding tests.
Moreover, though a goal of molecular taxonomy is to identify species without reference to living strains, the overlap of inter- and intraspecific cox1 sequence variation suggests that breeding tests on micronucleate species may be necessary in some instances.
Most of the named species of Tetrahymena with micronuclei are bona fide species as based on breeding tests, though there are a few, especially in the "americanis" clade, that need to be reexamined [ 25, 33].
Breeding tests confirmed that this phenotype was inherited in an autosomal-recessive fashion and the mutant strain was named deaf14.
For breeding tests, these females, together with their XX sibling controls, were mated at 6 weeks to MF1 males and offspring recorded daily.
The phenotype of WT, hCGβ+, and cabergoline-treated hCGβ+ females were analyzed in early adulthood (2 months of age), coincident with the age of the breeding tests (Fig. 2).
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