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The Flp transgene was segregated by a further breeding step.
The desired mice are produced with a mixed genetic background at low frequencies in the final breeding step.
An additional breeding step was needed to generate in the F2 the heterozygous reporter mice, carrying one miR-9-Sp allele and one tdTomato allele, both in the R26 locus.
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In particular, flowering-time genes are likely to have been involved in the domestication process to permit rice to grow in northern areas, although recent breeding steps may conceal older changes that occurred during rice domestication Fig. 2 Genome-wide restriction fragment length polymorphism (RFLP) patterns at 179 loci.
Two consecutive breeding steps were required.
First, only a limited number of plants were described in most cases, in contrast to the large number of events and subsequent breeding steps customary in a commercial program.
One further time consuming task included looking following chains of references to previous publications for human and animal model information such as mouse name, cross breeding steps applied and human subject information.
Thus, a saturated genetic map is considered as the first step for breeding by design and to reveal interested traits controlled by QTLs and genes.
Validation in these sites is desirable and would be a logical next step if breeding programs are started in these sites.
Getting an introduced gene to pass from parent to offspring is a first in a nonhuman primate and is a step toward breeding marmoset colonies with human disorders.
An important step in breeding maize for higher latitudes involved overriding this environmental control, creating maize plants that flower independently of day length.
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