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In order to avoid making mistake during all breeding stages, both in the field and laboratory, rabbit meat substrate was used to feed the maggots.
To investigate whether composition in aromatic plant species is repeatable across breeding stages, we tested whether similarity in nest composition was higher within than among nests.
The population was monitored all year round and breeding stages (nest building, egg-laying, incubation, feeding) were established for all couples breeding in the nest boxes.
Complementarily, we tested whether similarity was higher within than among breeding stages (egg-laying, incubation, chick rearing) (see below for details on similarity analyses).
The composition in aromatic plant species that female blue tits added in their nests within 24 h was repeatable across breeding stages.
Most seasonal breeders at mid- to high-latitudes use changes in day length (photoperiod) as a cue to transition from one life history stage to another, such as from non-breeding to breeding stages.
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The breeding stage involves two processes: crossover and mutation.
Breeding stage thus had an effect on different call-type combinations.
If the same pairs were sampled multiple times per breeding stage, mean values were calculated.
Here, we found an interaction between sex and breeding stage for all vocalisation types except for distance calls.
As recombinations at each breeding stage cannot be observed directly, it is of great interest to infer the ancestry of resulting DNA sequences.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com