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The results contribute to the understanding of the genetic bases for cold tolerance at the booting stage, and future cloning of the candidate gene will facilitate the molecular breeding of cold tolerance in rice.
The two candidate SSR makers should have immediate application for molecular breeding of cold tolerance in Elaeis guineensis.
Developing molecular markers related to cold tolerance would be helpful for molecular breeding of cold tolerant Elaeis guineensis.
These three candidate SSR makers would have immediate application for molecular breeding of cold tolerance in Elaeis guineensis.
Therefore, the EST-SSR markers developed based on sequences differentially expressed in response to cold stress have potential application for association analysis for molecular breeding of cold tolerance in Elaeis guineensis.
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These varieties may also be considered useful donors for further breeding of cold-tolerant varieties for the DPRK.
The future application of these QTL to MAS will significantly accelerate the breeding of cold-tolerant rice for temperate environments and high-altitude areas.
Therefore, the breeding of cold-tolerant varieties at the booting stage is an effective method to maintain high and stable yields in rice cultivation regions.
Certain up-regulated genes characterizing these processes are potential targets for breeding for cold and/or drought tolerance of woody plants.
In the context of increasing demand on crops for food and biofuel production, breeding for cold tolerance holds promises for enhanced yield and extended cultivation seasons.
For selection of appropriate parents for cold tolerance breeding, evaluation of cold tolerance at the reproductive stage is important.
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