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JAX and other labs making new mouse strains have long relied on a laborious multistep process that involves genetically altering mouse embryonic stem (ES) cells, injecting them into an embryo, and breeding multiple generations of animals.
In conventional breeding, multiple expressed traits are investigated during a whole growing period and after harvesting.
In pig breeding, multiple sire and dam lines are used, with a minimum of two lines (typically for crossbred sows) and often additional sire lines to produce a three-way or four-way cross finisher pig (Merks and De Vries 2002; Lutaaya et al. 2001).
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In an actual breeding program, multiple locations and possibly multiple years are needed so allelic effects are estimated across a set of environments representative of those in which the cultivar is grown in subsequent seasons.
There are many breeding systems which have been developed for this multiple breeding populations, such as sub-line breeding system, replicate population breeding system, multiple population breeding system etc.
Due to the complexity of breeding for multiple abiotic stresses, and to the large diversity within and among taxa and species of oilseed crops, the breeding process will have to be enabled by more complex models and genetic prediction methodologies.
We also simulated a breeding scheme, multiple restrictions, in which several of the restrictions that remove solutions from the solution space were imposed simultaneously.
Williams et al. (1995) compared the impact of two breeding strategies, multiple populations versus hierarchical, on loblolly pine genetic diversity using isozymes.
The fact that the positive allele for yield at this QTL is for the most part BAT477 but sometimes DOR364 indicates that one must consider the effect of substituting alleles when breeding for multiple environments.
Here, we describe the development of a novel mouse model for inducible expression of Prdm14 by targeting FS- Prdm14 to the ROSA26 locus and breeding to multiple Cre-recombinase transgenic lines.
Using a repeated-measures design, we followed individual females from pre-breeding, through multiple breeding attempts, and attempted to decrease PRL using the D2-receptor agonist, bromocriptine.
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