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Heterozygous B6 Cd151+/− females were crossed with FVB Cd151+/− males carrying the MMTV/PyMT transgene (PyMT Cd151+/− males, see Additional file 1: Figure S1 for breeding details) in order to generate the experimental F1 animals.
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Breeding for particular colors and patterns requires detailed knowledge of genetics.
Details of breeding strategies and genotyping are described in Supporting Information.
Since 2009, males have been monitored, banded and recorded in this area (see [ 40, 80] for details on breeding site and data acquisition).
No detailed selective breeding program has yet been reported.
Further detail on breeding and dosing of the 76 euploid and Ts65Dn animals used in morphometric analyses is provided in supplementary material Table S2.
The importance of genetic relationship between training and validation set is discussed in further detail in breeding scenario C. Prediction accuracy depends on the prediction problem that the breeder is attempting to address.
In his description of the WoDaaBe of Niger, Krätli details how their breeding system is devoted not only to ensuring genetic diversity, but also to ensuring that cattle behaviour necessary for survival, such as selective feeding on the most nutrient-rich grasses, is passed down from generation to generation (Krätli 2008).
Details on the breeding sites, geographic coordinates and sampling sizes for each ectoparasite species and seabird host are specified in Table S1 (Supplementary material).
Further technical details of the breeding system in this species are given in previous works [ 16, 18].
Figure 1 presents a compilation of tick count data from various studies of the 1980s on adaptation, production or heterosis [see Frisch and Vercoe (1984), Frisch (1987), Frisch et al. (2000) for details of environment, breeding programmes experimental designs and various analyses of data].
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