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Remarkably, germ-free mutants showed reduced mortality and some became capable of breeding (data not shown).
However, since the gender bias is not consistently observed in the SR/CR breeding (data not shown), the bias may alternatively stem from the knockout backgrounds.
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Then GABA-α6-tTA/tetO-NR2B double transgenic (simply tg hereafter) mice were produced and these mice showed normal growth, eating behavior, body weight, and breeding behaviors (data not shown).
This vaccine protects harbor seals (Phoca vitulina) and dogs against phocine distemper virus infection (2 ), which is closely related to CDV, and resulted in protective antibody levels to CDV in African wild dogs monitored at the beginning of this captive breeding program (data not shown).
The acetyl hydrolysis into acetic acid increased with severity of the pretreatment, and it maxed out at CSF of 1.98 and 2.16 for classical breeding varieties and industrial bagasse, respectively (data not shown).
Samples collected from Padre Island across all years clustered with high support (>0.924; data not shown) with samples collected from breeding grounds identified as F. p. tundrius/anatum.
Of the treated horses, seven are still active at the polo club and two are kept for breeding purposes and are still healthy to date (September 2012; data not shown).
Though IR2 itself could not distinguish Bph9 and Bph18 (data not shown), the combination of B9D and IR2 could facilitate MAS of Bph9 in BPH resistance breeding programs.
Breeding studies also indicate that Kv10.1−/− mice could interbreed, proving that they are fertile (Data not shown).
A sex-ratio distortion was also seen in mid-stage Alkbh1−/− embryos (E10 E12.5) after heterozygous breedings (17 litters), with 89% male and 60% female embryos present compared with wild-type embryos (data not shown).
Within breeding populations, it ranged between 0.34 and 0.84 for grain yield, 0.32 and 0.90 for anthesis date, and 0.31 and 0.71 for anthesis-silking interval (data not shown).
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