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Shelton was the kind of manager who could recite the details involved in every step of production, from the density of breeding cages to the number of birds processed per man-hour.
Timed-pregnant C57Bl/6J mice from the animal facility of the University Medical Center Hamburg-Eppendorf were housed individually in breeding cages at a 12 h light/12 h dark cycle and fed ad libitum.
Adult wild type, ERαKO and ERβKO animals selected for this study originated from litters of heterozygous ERα or ERβ breeding cages in a pure C57BL/6 genetic background.
Following surgery, Neosporin® (Pfizer, Morris Plains, NJ) was applied to each ear, and birds were monitored on a homeothermic blanket (Harvard apparatus Ltd., Edenbridge, UK) until recovery from anesthesia when they were returned to their parents in breeding cages.
To determine how much time juvenile birds spent singing relative to adults, 75d or >120d males from our aviary and >120d pair-bonded males from breeding cages were placed individually in sound attenuation chambers for 5 consecutive days and their songs were recorded during this time.
We checked breeding cages daily for births.
Similar(27)
Since it is a prolific breeder and spends much time rearing chicks, it needs a fairly large breeding cage in the aviary, even though its powers of flight are not exceptional.
For all breeding following the F2 generation, two males and two females were placed in a breeding cage.
Control non-stressed rats are tested in a breeding cage as stressed animals.
The pups were sacrificed by decapitation, and the F1 dam was immediately returned to the breeding cage.
Conventional pair breeding was performed, the plug was confirmed and then the male mouse was removed from the breeding cage after one day.
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