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Twenty early fourth instars larvae were introduced in insects breeding box containing 100 mL of water with each concentration.
In addition, each sub-module is composed of a first wall (FW), a breeding box with a seven-layer breeding zone (BZ), and side walls with a cooling path.
At hatching, we moved the experimental individuals and their carcass to a new breeding box.
Rats were initially divided into two groups: sham-operated rats (sham group, n = 8) were fed in a breeding box each with 4 rats normally and a permanent ligation of bilateral common carotid arteries (LBCCA) group (n = 72).
We then randomly assigned each brood to one of two maternal care treatments: either widowed females were removed from their breeding box around hatching (roughly 71 hr after pairing) or removal occurred 24 hr after hatching (roughly 95 hr after pairing).
The breeding box floats in the aquarium.
Similar(49)
Larvae in their dispersal stage were gently collected from breeding boxes and placed in eclosion boxes provided with moist soil.
Adult beetles were breed when they were between 2 and 3 weeks old in plastic breeding boxes (17 × 12 × 6 cm) filled two-thirds with moist soil with a mouse carcass.
Breeding boxes were kept at 22 ± 1 °C.
The pigs, under the control of a veterinary anesthesiologist, were recovered from general anesthesia and housed in standard breeding boxes.
As before, all males were removed from the breeding boxes ca. 53 hr later, just prior to larval hatching.
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