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The DNA fragment containing the transgene was injected into FVB/N blastocysts and two lines (H5 and H12) were selected for continued breeding and analysis.
How do the authors control for this in their breeding and analysis scheme?
D.E.K. has served as a consultant for Abbott Laboratories and Gilead Sciences, Inc. DS performed the arterial pressure and Na balance studies, SR performed the RNA analysis, SW performed the DNA analysis, RK developed and provided the Pax8-rtTA and LC-1 mice, KAS supervised the breeding and analysis of mouse genotypes, and DEK designed the study, analyzed the data, and supervised the project.
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We utilize genotyping information derived from DG to quantify genetic relationships among accessions in the sorghum germplasm collection (n = ~40,000), for marker-assisted breeding and pedigree analysis, genetic map construction, QTL analysis, map-based gene cloning and association studies.
Although these methods have generated numerous mutant alleles, large-scale genetic analysis in vertebrates is costly and both time- and labor-intensive including the breeding, maintainance and analysis of many fish over multiple generations.
From extensive breeding and genotyping analysis, we have found the knockout of Cdk2ap1 resulted in very high penetrating rate of embryonic lethality.
The high density lotus genetic map provides a framework for marker assisted breeding and QTL analysis.
Moneymaker is the standard introgression free tomato used in our group to make mapping populations for breeding and genetic analysis.
In general, we observed no statistically significant phenotypical changes in the mice during background breeding and SHIRPA analysis.
Saturated genetic linkage maps (< 1 cM between markers) are required for the efficient and effective deployment of markers in plant breeding and genomic analysis.
Founder mice entirely derived from female XO, heterozygous, or homozygous mutant B6 ES cells were directly available for intercross breeding and phenotypic analysis in vivo.
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