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In the first experiments, granulosa cells from Sarda breed ewes were freeze dried according the protocol described for sperm cells [4].
Peripheral blood lymphocytes were isolated from Assaf breed ewes through a Ficoll-Paque density gradient; the purity of the cells (∼80%) was assessed by an automatic cell counter (Pentra 60, ABX, France).
No cases of disease were recorded in breed ewes or the farm staff.
It was recorded in a farm with 115 small-tailed Han sheep, including 95 breed ewes and 20 lambs aged 1 to 5 months, located in northeast Nongan county, China (124°31' E, 44°55' N).
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This study involved seven sheep of the Assaf breed - six females (223, 292, 333, 336, 697 and 698) and one male (166) - and a Milchschaf breed ewe (368), ranging in age from 2 to 5 years.
Next week, the Manzanareses will escort the bred ewes, horses, dogs and assorted equipment to the lambing grounds west of Taos.
The two lines included an unselected control line of randomly bred ewes and a line that had been selected since 1976, based on the weight of lamb weaned.
Experimental animals were mature parous Greyface (cross between Scottish Blackface and Bluefaced/Border Leicester breeds) ewes 3 6 years old weighing 50 75 kg.
Through 5 years of herd life, values reported for cumulative number of lambs weaned from straightbred bred ewes within breedtype comparison studies were 4.17 [ 17], 3.88 [ 34], and 3.77 lambs [ 35].
Ram selection was obligatory between October 2004 and September 2005 for all sheep farmers with more than 10 breeding ewes (certain small breeds being exempted), and between September 2005 and June 2007 for all sheep farmers (including those with less than 10 breeding ewes).
Most commercial sheep today represent two-breed or three-breed crosses, with white-faced crossbred ewes preferred in the range areas and a black-faced sire, such as Suffolk or Hampshire, preferred for market lambs, which are either finished for slaughter or sold as breeding ewes.
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