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Even when herds were not dispersed or destroyed, a breed could be lost through indiscriminate breeding arising from a need for numbers.
At Geneva, such a robust new generation has been born that the breed could be perpetuated for years.
Some members of the breed could be noisy, and were described as "babbling" and making noise on the hunt in a similar fashion to hounds, while others were far quieter.
The Ardennes breed could be a direct descendent of the prehistoric Solutre horse, and is thought to be descended from the type of horse described by Julius Caesar in his Commentarii de Bello Gallico.
This high differentiation, particularly for the ESM breed, could be explained by a strong founder effect, genetic drift and differences in the choice of individuals made by breeders.
Taking all of this into account, it was considered that PRA locus for this breed could be close to the GALK1 marker.
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This showed that the sex of these five breeds could be easily and effectively determined using the PCR technique.
Results from the pedigree-based analysis showed that most Ne values ranged between 50 and 100 except the Shek-Ki breed which exhibited the smallest value (46) so that most breeds could be considered as safe from a conservation point of view.
We calculated allelic polymorphism rate, heterozygosity rate, genetic distances, linkage disequilibrium (LD), and ultimately performed clustering methods to test the hypothesis that the four individual breeds could be separated using the polymorphism pattern of the genotyping data collected.
The differences between breeds could be due to the difference in their genetic background.
Similarly, the occurrence of Arg563His in different breeds could be explained by adaptive selection.
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