During the second baseline blood collection, simultaneous breath samples were collected by having the subjects breathe into breath collection bags fitted with one-way valves.
Our secondary aim was to compare healthy and lung cancer groups when breath samples were collected with different collection setups.
If a child could not expire into the collection bag, breath samples were collected by applying a breath mask with two unidirectional valves connected to a breath bag.
In healthy subjects, end-expiratory breath samples were collected by asking them to fully breathe out into sample tubes through a straw.
Breath samples were collected at baseline and every 15 min postprandially until 4 h.
Breath samples were collected from 10 dialysis patients immediately before, during, and after a dialysis session.
[1-13C]Leucine was intravenously infused for 8 h and blood and breath samples were collected during the latter 2-h period of infusion.
On d 33, [13C] sodium bicarbonate was infused into one jugular vein for 6 h, and hourly breath samples were collected.
But Mr. McCulloch said cancer patients who had begun chemotherapy were excluded, smokers were included in both groups and the breath samples were collected in the same rooms on the same days.
Breath samples were collected by blowing gently into a 10 ml Exetainer (Labco, Buckinghamshire, UK) with a drinking straw and replacing the cap just before the end of exhalation.
Blood samples and expired breath samples were collected hourly during the 4 hour trial and vastus lateralis muscle biopsies obtained at 1 and 4 hours after tracer priming in the overnight fasted state.
