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In keeping with the observations presented by Pawlik, they also identified differentially expressed peaks between cancer and control fluids, but no significant differences between fluids from the healthy and cancerous breasts of the same patient.
Figure 7 presents the scatterplots of the ipsilateral and contralateral breasts of the same patient with fluorescence data acquired at different time points after the injection of contrast agent: 1 hour 30 minutes, 4 hours, 8 hours and 24 hours after injection.
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To further validate our findings of CXCL13 protein overexpression in human breast cancer and to localise the expression within a given tissue sample, we performed immunohistochemistry on breast cancer samples and autologous breast tissue samples of the same patients using lymph follicles as positive controls.
All women in the study group received an annual mammogram and physical examination of the breasts on the same day, or just a few days apart.
d and e MRI of the breast of the same patient in the Fig. above.
HMD and LMD tissue biopsies from the same breast of the same woman were analysed in a pairwise manner, which overcame potential confounding factors such as age, body mass index (BMI), menstrual cycle and metabolic activity.
Interestingly, two of the studies that detected different profiles in breast cancer patients versus healthy women reported a conservation of proteomic signatures within the fluids collected from the cancer-bearing and healthy contralateral breast of the same patient [ 65, 66].
The present study included control tissues from patients with no history of the disease as well as patients with benign breast disease, while the previous study looked at expression levels in tissue from the tumour-bearing breast of the same patients.
For this study, matched tissue pairs (n=55) of invasive breast cancer and corresponding physiological breast tissue of the same patient were analysed for their pp125FAK protein expression by Western blot analysis and immunohistochemistry as well as for RNA expression levels by RT PCR.
Because larger breasts generally have greater adipose tissue content, they also tend to have lower percent or proportion of density compared with smaller breasts with the same amount of dense area.
Six cases were tested for reproducibility where four slides were made from the breast tumor of the same patient.
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