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Breast tumour samples from women with a minimum follow-up of 5 years were assessed.
We received 155 breast tumour samples from the Breast Cancer Campaign Tissue Bank.
These results are then compared with those obtained on an independent dataset of normal and breast tumour samples from The Cancer Genome Atlas (TCGA) [ 24].
In this study, we evaluated the methylation patterns of ESR1 and two CpG islands in the CXCL12 gene in breast tumour samples from Brazilian women.
To test this hypothesis, we examined a series of ER-positive breast tumour samples from 55 patients that had received tamoxifen therapy and a minimum of 6-year follow-up was available (supplementary Table S1).
Oestrogen receptor mRNA was detected in 90% of the 60 primary breast tumour samples from patients not receiving endocrine therapy at the time of surgery, whereas the pNR-2 RNA was detected in 57%.
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Thus, we applied it to a fresh frozen breast tumour sample from Dataset1 (Dedeurwaerder et al., 2011) (Fig. 1A).
All 76 breast tumour samples were from primary breast carcinomas.
Formalin-fixed paraffin-embedded breast tumour samples obtained from 86 Centre Léon Bérard (French agreement no. DC-2008 99) DC-2008 99iagnosed in 1998 with invasive breast cancer were used.
The selected breast tumour samples were obtained from paraffin blocks from our files.
The breast tissue series used for microarray profiling consisted of 66 FFPE familial breast tumour samples, including 13 from BRCA1 mutation carriers, 10 from BRCA2 mutation carriers and 43 BRCAX tumours, 10 sporadic breast carcinomas, and 4 normal breast tissue samples.
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