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The Prosigna assay is based on PAM50, a 50-gene microarray profile originally developed for research purposes to assess the phenotypic diversity of breast tumors and corresponding diversity in gene expression [ 14, 15].
Recently, Jensen et al. [31] assessed the PIK3CA mutation status in both primary breast tumors and corresponding metastases and showed a high discrepancy between primary tumors and metastases with either a gain or a loss of PIK3CA mutation in metastases.
Global miRNA expression profiling was performed on 47 tumor samples from 14 patients with paired samples from primary breast tumors and corresponding lymph node and distant metastases using LNA-enhanced miRNA microarrays.
Twelve matched pairs of primary breast tumors and corresponding brain metastases, nine non-breast brain metastases and 26 tumor samples from the six autopsy cases were subjected to primer extension and MALDI-TOF mass spectrometry using the OncoCarta® Panel Assay v1.0 (Sequenom Inc., San Diego, California, USA) of 238 mutations in 19 oncogenes [ 26].
The samples of breast tumors and corresponding adjacent normal-appearing tissues (from tissues located at least 3 cm away from the site at which the tumor was sampled) came from 80 patients who had undergone surgery at The University of Texas MD Anderson Cancer Center in 2004 or 2005 and 10 patients who had been diagnosed with breast cancer between 1995 and 2003.
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Samples of breast tumor and corresponding adjacent normal-appearing tissue (located at least 2 cm away from the site at which the tumor was sampled) were collected from 215 patients diagnosed with a ductal infiltrative breast carcinoma.
Methylation status of 40 tumor suppressor genes was compared between 53 primary breast tumors and their corresponding metastases to brain, lung, liver, or skin.
Data-mining of microarray expression data from primary breast tumors and the corresponding clinical data showed high ZNF217 expression correlates with shorter overall survival and relapse-free survival [ 3].
This study aimed to compare expression levels of several membrane proteins between primary breast tumors and their corresponding distant metastases in view of their potential applicability for molecular imaging and drug targeting.
The expression of CD138 on primary breast tumors and in the corresponding invaded lymph node has been evaluated.
In order to compare the RNA expression of some NE tumor samples (metastatic liver, pancreas and breast tumors) with non-neuroendocrine tumors and corresponding normal tissues, commercial total RNAs from 2 non-neuroendocrine tumors (pancreas and breast tumors) and from the 2 corresponding normal tissues were used in a second experiment.
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