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In this study, we investigated the localization of tamoxifen in five ER-positive and five ER-negative breast tumor sections using an experimental model by MALDI-MSI.
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Immunohistochemistry was conducted on whole tumor sections using HNF1β.
Additionally, Additional file 3: Figure S3 presents all breast tumor sections analyzed by MALDI-MSI using identical experimental parameters.
Our results were further validated by using clinical samples of breast tumor sections obtained from human patients with different grades of cancer.
Instead, the selected regions of interests (ROIs) of tumor cell dense and stroma areas were compared based on careful annotations of these regions by a trained pathologist (see "Localization of tamoxifen in human breast tumor sections" section).
Human breast tumor sections for immunofluorescence were obtained courtesy of the Breast Cancer Functional Genomics Group at McGill University.
Specifically, the study considered immunoperoxidase-stained tumor infiltrating lymphocytes within breast tumor specimens, using the number of immunostained pixels within tissue sections to determine cellular density and number.
This paper presents the modeling of breast tumor imaging process using wire mesh collimator gamma camera.
A human breast tumor specimen was used because breast tumors have been reported to have positive 53BP1 expression [ 23, 24]. 5 μm-thick consecutive sections of formalin-fixed, paraffin-embedded breast cancer tissue were de-paraffinized and rehydrated as described above.
4T1 mouse breast tumor cells were used as targets.
Frozen tumors were sectioned using a cryostat microtome.
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