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Expression levels of miR-135a were detected from 30 human primary breast tumor samples using poly (A -RT-PCR, A -RT-PCRusly described [ 31].
A recent report that analyzed FAK expression in 98 breast tumor samples using TMA analysis did not show a significant association with FAK and prognostic indicators in breast cancer [ 15].
Five main molecular subtypes (luminal A, luminal B, basal, ERBB2-overexpressing, and normal-like) have been identified by gene expression profiling of breast tumor samples using an intrinsic set of ~500 genes [ 40, 41].
We tested 11 human breast tumor samples using: (a) FF RNAs by microarray, mRNA-Seq, Ribo-Zero-Seq and DSN-Seq (Duplex-Specific Nuclease) and (b) FFPE RNAs by Ribo-Zero-Seq and DSN-Seq.
We constructed RNA-Seq libraries using eleven UNC breast tumor samples using different sample preparation protocols including: (a) FF RNA samples by mRNA-Seq, Ribo-Zero-Seq and DSN-Seq and (b) FFPE samples by Ribo-Zero-Seq and DSN-Seq.
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The breast tumor samples used were invasive ductal carcinoma and mostly (> 90%) ER-positive.
Total RNA was extracted from breast tumor samples by using the acid-phenol guanidium method.
Three-hundred and 44 human breast tumor samples were used from a collection at the Erasmus Medical Center (Rotterdam, Netherlands), which were primarily from one breast cancer genetic expression study [ 5] and later supplemented by 58 additional ER- samples [ 19].
The human tumor samples used in this study were collected from breast cancer patients at time of surgery.
Similarly, we also evaluated the correlation between all the cancer cell lines in CCLE and breast tumor sample groups, using the copy number information and gene expression profiles (supplementary Tables 4 and 5).
The differential expression of genes was determined using the two-tailed Student's t-test, and the differential expression of H19 in the breast tumor samples was determined using the two-sided Mann–Whitney rank test.
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