Sentence examples for breast tissues derived from from inspiring English sources
Second, we investigated if the pattern of gene expression in normal breast tissues derived from breast cancer patients was associated with clinical or pathological features of the corresponding cancer.
To avoid epigenetic variations due to age, reproductive state and/or cancer stage, we pooled together, whenever possible, DNA of normal breast tissues derived from comparable donors (same age frame, tumor stage, estrogen receptor/progesterone receptor status, and so forth).
Whole tissue sections from 15 pure in situ breast carcinomas (five low, five intermediate and five high nuclear grade), 101 invasive breast carcinomas and 14 histologically normal breast tissues derived from reduction mammoplasties, together with microarrayed tumour cores from 253 breast carcinomas, were collected from patients undergoing surgery at the John Radcliffe Hospital, Oxford, UK.
Fifteen breast cancer specimens with paired DNA from adjacent normal breast tissue derived from a part of the breast resectate that was devoid of macroscopic tumor were analyzed.
We performed a comprehensive and integrative analysis of SEPT9 gene amplification, expression at the mRNA and protein levels, and epigenetic profiling using a panel of human breast cell lines, tissues from breast cancer patients with premalignant lesions and primary adenocarcinomas, and normal breast tissue derived from reduction mammoplasty patients.
Breast tissue derived from reduction mammoplasties was cut up manually into small pieces (approximately 0.5 cm cubed) and digested overnight at 37°C in a shaking incubator with 0.5 1 mg/ml collagenase (Type I; Sigma, Poole, UK) in phenol-red free DMEM (Gibco, Paisley, UK) supplemented with 5% charcoal-treated serum.
In past years, a large number of clinical studies have described Met-receptor overexpression and pathway hyperactivation in tissues derived from breast cancer patients, and have found a strong relationship between high HGF/Met signaling and tumor progression (Table 1).
We therefore investigated short-term effects of chemotherapy on tumor stroma ex vivo in primary tissues derived from newly diagnosed breast cancer and lung cancer specimens and in isolated CAFs from breast and lung.
