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To confirm the tumor suppressor function of BRCC2, we first examined the levels of BRCC2 in breast cancer samples and matched normal breast tissue samples using western blotting.
Total RNA was isolated from 50-100 mg breast tissue samples using TRI-reagent (Ambion Inc., Austin, TX, USA) following the manufacturer's instructions.
The luminal A/luminal B distinction seems also less obvious in a recent study [ 6] that classified 83 breast tissue samples using a reduced set of genes, which included 120 genes from the later 500-gene set [ 4].
About 1 μg DNA was isolated from approximately 10,000 cells microdissected from metastatic tumor and normal (glandular and stromal) breast tissue samples, using a modified method of DNA extraction from archival tissues.
In the present study, we present a systematic expression analysis of ERH in a panel of breast cancer cell lines and malignant and normal human breast tissue samples using Northern blot, quantitative RT-PCR and non-radioisotopic RNA ISH.
In order to analyse the expression of the diverse Rho species on the level of the mRNA, total RNA was isolated from breast tissue samples using the Quiagen RNA extraction kit (Quiagen, Hilden, Germany).
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Conversely, no FRA-1 protein was detectable in 6 normal breast tissue samples used as controls.
The breast tissue samples used in this study were obtained from the Department of Surgery, St Vincent's University Hospital, Dublin and the Department of Pathology, Mater Private Hospital, Dublin.
bMetastatic potential verified through of spontaneous metastasis assays in immune deficient mice The tumor and adjacent non-tumor breast tissue samples used in this study were provided by the Tumor Bank of the A.C. Camargo Cancer Hospital and Alfredo Abrão Cancer Hospital.
RFIB comprises the removal of an intact breast tissue sample using a high-frequency electrosurgical cutting device.
Quantification of OPG staining in breast cancer tissue samples using Image J. (PPT 121 kb).
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