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We analyzed expression of Rab2A, Pin1, and ALDH1, a marker for stem and progenitor cells as well as BCSCs (Ginestier et al., 2007), in normal and cancerous breast tissue arrays using immunohistochemistry. Pin1 and Rab2A were undetectable or low in all 24 human normal breast tissue, but their expression was dramatically increased in many of 65 human breast cancer tissue.
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It was, therefore, of interest to determine the expression of Lewisy/b in a large series of breast cancer tissue arrays using the SC101 mAb.
The cancer tissue array used was ones of AccuMax array (Petagen Inc, Seoul, Korea).
Previously, we detected PTK6 expression in a normal mammary gland human breast tissue array, as well as in a human breast tumor array using immunohistochemistry. Phosphorylation of tyrosine residue 342 in the PTK6 activation loop promotes its activation.
Sections (10 μm thick) were cut from frozen tissue and from breast tissue array paraffin blocks.
To study the distribution of JMJD6 protein levels, we immunohistochemically (IHC) stained an 81-breast tumor tissue array by using a JMJD6-specific antibody.
A human breast cancer test tissue array was used with self-matching or unmatched normal adjacent tissues (US Biomax Inc BR2411) as a positive control.
We evaluated the expression of ERLIN2 in normal and cancerous human breast tissues using immunohisyochemistry (IHC) in breast cancer tissue arrays.
Results from breast cancer tissue arrays demonstrate a higher NURR1 expression in the normal breast epithelium compared to breast carcinoma cells (p ≤ 0.05).
Material I was a breast cancer progression tissue array constructed by using cutting-edge matrix assembly [ 35] and represented 180 unmatched patient specimens, including 40 normal breast tissues, 20 ductal carcinoma in situ (DCIS), 100 invasive ductal carcinomas (IDCs), and 20 lymph node breast cancer metastases [ 22].
Pathway Studio9 software (Elsevier, Ariadine Genomics, MD) was used to evaluate NILCO and its targets' interactions in breast cancer tissue arrays.
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