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The IFNγ receptor α was localized in the epithelial cell cytoplasm, in the three breast groups.
A comparative semiquantitative immunohistochemical study between the three breast groups (fibrocystic lesions, in situ tumours and infiltrating tumours) was performed.
Immunohistochemical and semiquantitative study of IFNγ, its receptors types (IFNγ-Rα and IFNγ-Rβ), cell proliferation (proliferating cell nuclear antigen, also named PCNA), and apoptosis (TUNEL method) was carried between the three breast groups (fibrocystic lesions, in situ tumors and infiltrating tumors).
Comparison of optical density band's revealed significant differences (p ≤ 0.05) between three breast groups: the highest optical density was found in in situ carcinomas (IFNγ and IFNγ-Rα); no significant differences were found to IFNγ-Rβ between the three sample groups (Table 1).
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The only exception was the breast group, who reported worse MCS than the normative data (P<0.05).
In the M/D breast group, in total 157 594 person years were accumulated.
From the average values obtained for each breast, the means ± SD for each breast group were calculated.
Personal experience took priority in the breast group, while information giving/seeking was ranked first in the prostate group.
Experienced screening radiologists prospectively evaluated each case, documenting mammographic sign, size and classification according to the RCR breast group guidelines.
In the F breast group, in total 158 950 person years were accumulated for the BC mortality analysis, Table 2.
It should be noted that the proliferation signature was not evaluated in the Normal-like breast group because this group included control samples and few cancer samples.
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