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All exons of the SLC25A43 gene were screened in HER2-positve breast cancer samples using single-strand conformation polymorphism (SSCP).
Total RNA was isolated from breast cancer cell lines and homogenised breast cancer samples using the AB gene Total RNA Isolation Reagent Advanced Biotechnologies Ltd.., Epsom, Surrey, UK).
Now, we have extended the study of the rate of Amp13q34 to a larger cohort of 414 familial and sporadic breast cancer samples using FISH technique.
Subsequently, these 17 genes were further evaluated for differential expression in publicly available expression datasets of clinical breast cancer samples using the Oncomine database [ 35].
It is important that the screening of breast cancer samples using whole genome platforms continues in order to design better prognostic signatures.
To create the standard dilution series templates, ER and HER2 amplicons were cloned from pooled samples of known ER- and HER2-positive breast cancer samples using Invitrogen TOPO TA cloning kit.
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The 28 human breast cancer samples used in this study are listed in Figure 1.
Clinical information and iRAS scores for all breast cancer samples used in this analysis.
In contrast, all of the 12 breast cancer samples used as positive controls had a clustered HER-2/neu gene amplification.
This could in part result from the small number of CHEK2*1100delC breast cancer samples used in this study.
In addition, the present study examined almost two times more breast cancer samples, used each tumor in triplicate, and the scoring system of triplicate samples included not only the average intensity but also the extent of staining, allowing for more accurate analysis.
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