Sentence examples for breaks were labelled by from inspiring English sources
DNA strand breaks were labelled by polymerisation of fluorescein-dUTP to the 3′-OH sites, catalysed by terminal deoxynucleotidyl transferase (TdT), revealed by antifluorescein Fab fragments conjugated with alkaline phosphatase (Converter-AP), and visualised using Fast Red DAKOO, Glostrup, Denmark).
DNA strand breaks were labeled by incubating cells with 50 μl TUNEL reaction mixture containing TdT and FITC-dUTP in binding buffer for 60 min at 37°C.
After 3 days of siRNA transfection followed by 24-h gentamicin treatment, DNA strand breaks were labeled by TUNEL reaction by using In Situ Cell Detection Kit (Roche Applied Science, Indianapolis, IN, USA), and detected by confocal microscopy.
DNA breaks were labeled sequentially by TdT and DIG-labeled deoxyuridine triphosphate and then were incubated in blocking buffer, biotinylated antidigoxin IgG, streptavidin biotin complex (SABC), and diaminobenzidine (DAB, for color development) in turns.
DNA strand breaks were labeled with fluorescein-conjugated dUTP using terminal deoxynucleotide transferase (Boehringer Mannheim) as recommended by the vendor and visualized by fluorescence microscopy (TUNEL) as described below.
Briefly, DNA strand breaks were labeled with fluorescein-12-dUTP.
Free 3'-OH termini of DNA strand breaks, generated by DNase preferentially during apoptotic cell death, and were labelled by incorporation of fluorescein-dUTP by terminal transferase reaction.
Some evidence of TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling) staining has been reported (Smith et al., 2005), although this could be apoptosis or necrosis as DNA strand breaks can be labelled by this technique in either mode of cell death.
The DNA strand breaks in apoptotic cells are labelled by attaching biotin-or digoxygenin conjugated dUTP in a reaction catalyzed by exogenous terminal deoxynucleptidyl transferase (TdT-assay) or DNA polymerase.
So this mix of promise kept and promise broken is labeled a compromise.
