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When you've got tens of thousands of people tuned in and waiting for news the very instant it breaks, issues that might otherwise seem trivial can bring your entire operation to a grinding halt.
Various technical issues, for example problems due to formalin fixation-induced RNA strand breaks, issues of data validation and comparability with RT-qPCR data were extensively discussed in previous publications [ 25, 26].
Immunity is a deal-breaking issue for the United States.
"Debt is the make-or-break issue for Iraq".
Afghanistan is a make-or-break issue for NATO.
With water and land, energy has become a make-or-break issue.
But command of policy is not a make-or-break issue.
"Members frequently say the cost of energy is a make-or-break issue," he says.
"Educating the young is the make or break issue in this country.
That in itself would be a deal-breaking issue for Liberty.
Both sides agree that minimums are the make-or-break issue.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com