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The double-strand breaks are processed to ssDNA, on which RecA forms filaments.
Recent work indicates that topoisomerase II-mediated DNA strand breaks are processed and eventually resected by an alternative nonhomologous end joining pathway.
Furthermore, since somatic cells may spend a long time in the G1 phase of the cell cycle, when double strand breaks are processed by non-homologous end joining (NHEJ), transposition may cause a net decrease in the number of transposon copies in the event of a failure to reintegrate.
This difference between these yeast species and vertebrate species may be due to differences in the manner in which double-stranded DNA breaks are processed in these taxa because, in the yeasts, these breaks are quickly processed into single-stranded DNA.
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In HDR, the DSB break is processed to a 3' single-stranded overhang and a different set of repair proteins stimulate strand invasion by a donor homologous template, followed by repair [ 8].
The most common cellular signals activating the SOS response are regions of single-stranded DNA (ssDNA), arising from stalled replication forks or double-strand breaks, which are processed by DNA helicase to separate the two DNA strands.
There is a considerable linguistic debate on whether phrasal verbs (e.g., turn up, break down) are processed as two separate words connected by a syntactic rule or whether they form a single lexical unit.
Our results show that some breaks must be processed at approximately this rate.
A break would be processed by RecBCD to create 3' ends which prime synthesis from a homologous DNA molecule.
Therefore, the majority of breaks seem to be processed considerably more slowly.
DNA double-strand breaks (DSBs) can be processed by the Mre11 Rad50 Nbs1 (MRN) complex, which is essential to promote ataxia telangiectasia-mutated (ATM) activation.
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