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Because the breaks are generated by a restriction endonuclease, it is expected that the damage will be more uniform than the damage generated by a DNA damaging agent such as X- or γ-irradiation.
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Briefly, a double-stranded break was generated by injecting a plasmid-expressing Mos1 transposase into a strain with a Mos1 transposon insertion in a ptrn-1 intron (ttTi21011 ).
Interestingly, our results suggest that the molecular events by which DNA breaks are generated in PHA-stimulated and non-stimulated cells are quite different.
We first interpolate break points, which are generated by computing the offset surface driving plane intersection curve reflecting the curvature, by a planar cubic B-spline curve.
A strong indication for such a repair system comes from the fact that the saturation of mitochondrial DSB repair machinery by a mitochondrially targeted endonuclease results in deleted mtDNA molecules that – unlike mtDNA deletions in healthy heart – do not have homology in the break-points, indicating that they are generated by non-homologous end joining [24].
Typically, the TSDs of cut-and-paste transposons arise from the staggered joining of the 3'OH transposon ends, which are generated by double-strand breaks at the ends of the transposon.
In analogy to PAMPs, sterile inflammation is triggered by damage-associated molecular patterns (DAMPs), which are generated by tissue injury or break down [4].
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(1) Double-strand breaks can be generated by endogenous sources such as reactive oxygen species produced during cellular metabolic processes and replication-associated errors, as well as by exogenous sources including ionizing radiation and chemotherapeutic agents.
To assure that only L1 was broken, stress risers were generated by cutting the superior endplate and lamina of the first lumbar vertebra prior to impaction.
The green triangle indicates the location of the introduced lesion, and the arrow indicates the 5′ single-stranded break that would be generated by BER.
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