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Breakpoints were observed for the implantation doses of 0.6 × 1015 and 4.4 × 1015 ions/cm2 for silver and gold ions, respectively.
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Tightly clustered strain- and sex-specific breakpoints are observed for both male and female meiosis across all chromosomes.
As well, no common position for breakpoints was observed, thus confirming the primary status of the ovarian tumors (Table 3).
It is interesting to note that the enrichment of Alus is observed even when the interval between probes is large, under which circumstance one would expect the non-breakpoint sequences to dilute any effect due to enrichment at the breakpoints (as was observed for LINEs).
However, microhomologies at breakpoints have also been observed for two other mechanisms involved in the generation of copy number variation: microhomology-mediated end joining (MMEJ) and microhomology-mediated break-induced replication (MMBIR).
A ρ value is observed for each inferred breakpoint in an alignment, so long as that breakpoint is associated with a domon boundary (and hence with a domon) annotated in at least one of the aligned sequences.
Indeed, over the sets of single-copy genes in these 144 prokaryotic genomes, gene sets with observed recombination breakpoints are about 50% more frequent than those of inferred lateral origin for which no breakpoint is observed, suggesting that LGT more commonly interrupts genes than preserves them intact.
Another breakpoint was observed at NAALAD2 gene in KYSE510, and it was located within FRA11F.
A similar pattern was observed for 227 independent rearranged copies, including the variable position of the breakpoints (Fig. 3c).
No band was observed for the EF animals; whereas, all LF animals showed the 78 bp band corresponding to the breakpoint junction.
High levels of linkage disequilibrium were observed within all 11 breakpoint regions as well as between the ends of most proximal and distal breakpoints.
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