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The breakpoint value can be seen as a measure for the rewarding value of the reinforcer [61], and breakpoints were found to decrease with decreasing nutritional value of a food reward whereas they increase during food deprivation [61], [62].
The integration site breakpoints were found to be identical in each bird, suggesting that they likely occurred prior to domestication.
In our study, 20% and 22% genome breakpoints were found to be located in the neighborhood of common fragile sites in MCF-7 and MDA-MB-231, respectively.
A total of 27 breakpoints were found to be shared between chicken and opossum but were not observed in any other species, suggesting that these constitute evolutionary breakpoints that occurred in the eutherian common ancestor (Additional file 2).
Deletion breakpoints were found to be of three basic types: clean breaks, breaks with one or more bases that could be assigned to either side of the breakpoint ("ambiguous" breaks), and breaks with one or more bases of inserted material ("insertion" breaks).
Both breakpoints were found to be enriched for low copy repeats, which, according to the Segmental Duplication Database (http://humanparalogy.gs.washington.edu/), share a 49.9 kb segment with 95% sequence similarity (no.9417), and the proximal breakpoint coincided with published DNA copy number variants.
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The 1q41 breakpoint was found to disrupt USH2A, a gene that is mutated in the autosomal recessive disorder Usher syndrome 2A (OMIM 276901).
When a recombination breakpoint was found to be significant, the alignment was broken into two or more gene fragments.
We noted the chr5 breakpoint was found to disrupt the first intron of a previously uncharacterized gene, UBE2QL1, so that the 5′ region, exon 1, and part of intron 1 were translocated onto chr19.
Of the 678 gene sets that yielded observable recombination breakpoints, 215 were found to be not topologically discordant with the MRP tree.
A total of 41 breakpoint intervals were found to be shared by these species, whereas 323 breakpoint regions were unique to the species considered.
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