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All microbiology laboratories used MIC breakpoints established by the NCCLS.
Clinical categorization was done using the 2013 breakpoints established by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) [ 9].
Breakpoints established by the Clinical and Laboratory Standards Institute for the microbroth dilution test on aerobic bacteria were used to interpret MICs [ 19, 20].
MICs of tigecycline were interpreted according to breakpoints established by the US Food and Drug Administration (Silver Spring, MD, USA) (www.rxlist.com/tygacil-drug.htm).
Our microbiology laboratory services performed antimicrobial susceptibility of isolates using a non-automated agar dilution method according to breakpoints established by the Clinical and Laboratory Standards Institute and in publication between 2008 and 2010 [ 20- 22].
Microbiology laboratories performed antimicrobial susceptibility testing of isolates using disk diffusion or automated testing methods according to guidelines and breakpoints established by the Clinical Laboratory and Standards Institute (CLSI) [ 16] and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) [ 17].
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Susceptibility interpretations for other antimicrobial drugs were based also on breakpoints established by NCCLS (24).
However, using EUCAST breakpoints established for Enterobacteriaceae (S ≤ 1 mg/L, R > 2 mg/L) only 14% and 18%, while with breakpoints established by USFDA for Enterobacteriaceae (S ≤ 2 mg/L, R > 8 mg/L) only 10% and 4% of the isolates were non-susceptible, respectively.
MIC breakpoints have recently been established by the Clinical and Laboratory Standards Institute for N. meningitidis (14).
Antibiotic susceptibility testing was performed and interpreted using guidelines and interpretive breakpoints for susceptible, intermediate, and resistant categories established by the Clinical and Laboratory Standards Institute (CLSI).
Although ethambutol and rifampin are useful clinically in cases of Mycobacterium avium complex infections [ 5], breakpoints for determining susceptibility and resistance have not been established by the Clinical and laboratory standards institute M24-A2 (CLSI M24-A2) [ 6].
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