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The effect of cell dissociation by trypsin-EDTA could be mediated through the physical breaking of cell:cell contacts or via specific effects of trypsin and/or EDTA.
These data suggest that the physical breaking of cell:cell contacts, rather than trypsin or EDTA mediated signaling, is regulating lineage choice.
CTL-mediated killing of endothelial cells indicated breaking of peripheral immune tolerance against this self antigen, resulting in markedly reduced dissemination of spontaneous and experimental pulmonary metastases.
Drugs of cancer based upon ionizing radiation or chemotherapeutic treatment may affect breaking of DNA double strand in cell.
It is possible that the tension generated by engagement of the actin-clutch is suddenly released due to a breaking of the cell-cell adhesion.
If everyone is moving the c-agent will have the option of breaking into neighboring cells also, even when the resident have him in his long term memory.
The loss of tight junctions and associated cell-polarity is also likely to be tumor promoting and might also be an early step in tumor initiation, leading to an ability of kidney epithelial cells to break free of cell-cell contact restraints and to proliferate.
This can be associated only with the break of cell walls and leakage of the intracellular fluid.
This paper lead to the conclusion that the break of cell walls may take place for lower pressure at lower temperatures (5 °C), already at 200 300 MPa.
All these may suggest that the action of pressure leading to the "cold pasteurization" phenomenon for commercially most often used pressures P = 400 600 MPa is associated not only with the break of cell walls but also the influence of intracellular structures and pressure-induced denaturation.
That is, the c-agent moves to a neighboring cell and the program gives an option of breaking into the cell.
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CEO of Professional Science Editing for Scientists @ prosciediting.com