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Furthermore, the protein smear found associated with NB, accompanied by the fading of the three prominent bands for example, could represent the result of proteolytic breakdown of serum proteins following prolonged incubation at 37°C.
For these experiments, we chose the two γ-irradiation doses of 30 kGy and 50 kGy, both of which have been shown to induce extensive breakdown of serum proteins.
Thus, both the inhibitory and seeding properties of serum appear to have been left largely intact following γ-irradiation, despite the extensive breakdown of serum proteins seen associated with the irradiation treatment.
Specifically, at the sterilizing γ-dose of 30 kGy used by all the NB proponents to date, there is considerable breakdown of serum proteins that can be reproduced and confirmed with the purified proteins albumin and fetuin-A.
Taken together, our results indicate that, for both FBS and HS, γ-irradiation results in significant breakdown of serum proteins, and, yet, this breakdown leaves largely intact the ability of the protein fragments or aggregates to bind to nascent crystals prepared from the addition of supersaturating amounts of calcium and phosphate ions.
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Ultimately, skeletal muscle breakdown, elevation of serum creatine kinase and hyperkalemia resulting in cardiac arrest, disseminated intravascular coagulation, and pulmonary and cerebral edema may be potentially fatal complications [ 56].
Human liver glycogen phosphorylase (HLGP) catalyzes the breakdown of glycogen to maintain serum glucose levels and is a therapeutic target for diabetes.
The level of serum cotinine, a breakdown product of nicotine, was evaluated.
No differences in muscle mass, markers of protein breakdown or serum creatine phosho-kinase were observed, except for a blunted increase in fat free mass in the glutamine group which led to a greater increase in fat mass percentage.
Intraoperative 360° laser, as well as cryopexy, might cause the breakdown of the blood retinal barrier with leakage of serum proteins into the intraocular fluids [ 32].
These abnormalities in bone function are reflected in abnormal levels of serum and urinary collagen breakdown products, which may therefore be exploited as biochemical markers to monitor progress of the disease and the effectiveness of bisphosphonate therapy.
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