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For example, we exposed only one sample at a time to the microscope light source and used the shortest exposure time possible to avoid introducing variation due to breakdown of the dye.
Besides the undesirable color, breakdown products of dyes also exhibit a mutagenic or carcinogenic effect on human beings and their ingestion can cause severe damage to organisms.
Akar and Özcan (2009) reported that the breakdown products of dyes are dangerous and cause serious health problems to both humans and animals.
Although there are numerous reports on the use of peroxidases for the degradation of organo-pollutants, studies that show detailed mechanistic pathway of dye breakdown are very few.
Discolouration of the dyed textiles was also observed during accelerated ageing due to the breakdown of the iron-tannate dye complex resulting in colours of cellulosic textiles being comparable to the colours of naturally aged cellulosic museum objects.
A thermal breakdown of the chemicals or dyes could potentially release toxic or even carcinogenic compounds without any detectable smell or flavor.
These results are explained by the breakdown of the blue-black iron-tannate dye complex with thermal ageing as previously described [6, 7] (see also Additional file 1), which has been observed with model iron gall inks on paper and traditionally dyed New Zealand flax on ageing [6, 32].
(237ml) bottle of dye.
Purchase the appropriate type of dye.
When dyes are accumulated in excess on the surface of the fungal hyphae, they might start exhibiting toxic reactions like breakdown of the hyphae structure as indicated by growth inhibition that results in leaching of dyes back into the assay medium.
A significant uptake of Evans Blue (EB) dye was detected in the ipsilateral cortex of VEH-treated animals at 10 h after MCAO, indicating the breakdown of BBB.
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