Fusion of an autophagosome with endolysosomal compartments leads to the breakdown of cargoes by acidic hydrolases, resulting in degradation and recycling.
The breakdown of autophagic cargo generates basic biochemical building blocks, such as fatty acids and amino acids, which can be exported back to the cytosol for reuse.
Interaction of syntaxin 17 with the HOPS (homotypic fusion and vacuole protein sorting) tethering complex promotes the fusion of autophagosomes with lysosomes, where breakdown of autophagic cargo takes place [ 25, 26].
This is a prerequisite for the subsequent proteolytic breakdown of autophagic cargo (Takeshige et al., 1992; Yang et al., 2006) and depends on vacuolar acidification, the catabolic activity of Pep4 and Prb1 and the lipase Atg15 (Teter et al., 2000; Epple et al., 2001).
Evaluation of the autophagic flux, a complete process of autophagy including the delivery of cargo to lysosomes, via its fusion with autophagosomes or amphisomes and its subsequent breakdown and recycling, [ 56] is important to determine whether drugs and/or surgical techniques truly affect autophagy.
"All kinds of cargo, everything.
It carried 2.4 tonnes of cargo.
Ariel Foxman, editor in chief of Cargo, disagrees.
Curiously, there were no signs of cargo.
There are three cargo box lengths and two styles of cargo boxes, Styleside and Flareside.
