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Execution times varied from less than one minute to more than two days, depending on the values for chain length, chain number, and breakdown constraints.
The chainbreaker routine (Figure 2B) determines whether a newly created chain (step 6) is acceptable by subjecting the chain to simulated digestions and comparing the released disaccharides with breakdown constraints.
The disaccharides released in each of the first two digestion steps are compared with the experimental products of sequential digestion, and if they fall within the allowable limits (breakdown constraints), the chain is designated as a successful chain (step 11).
Three groups (D0A0, D0A6, and D2S0) in Table 2 were chosen for comparison with the simulated disaccharide release, and practical tolerances for the breakdown constraints were set at D0A0 ±2% 4%, D0A6 ±20%, and D2S0 ±0% for each of the first two digestion steps.
Similar(56)
One model of CD suggests that it is initiated in genetically susceptible individuals by an infectious agent capable of triggering a breakdown in the regulatory constraints on the mucosal immune system and leads to an immune mediated tissue injury.
3– 5 During humanitarian crises, the feasibility of such tools is a major concern, given the demands placed on overstretched delivery agencies, operational constraints, the breakdown of social and public health networks, and the types of refugee shelter available.
Because of the probabilistic nature of the machine breakdowns, a set of chance constraints have been introduced.
Economic constraints, lack of security, breakdown of social structures, violence, loss of loved ones, and direct exposure to violent acts deeply affect children, who might not yet have the skills to cope with or even understand the situation.
If we had to speculate, we would say that the compact nature of tunicate genomes has allowed for a breakdown of colinearity, given the lack of constraints imposed by the long-range enhancers mentioned by the reviewers.
These constraints guarantee that the number of breakdowns for each machine never exceeds a predefined percentile.
The downsizing of CMOS technology into the decananometer range has called for the redesign of the ESD protection devices because of the constraints of lower operation voltage and smaller breakdown voltage of the ultrathin gate oxide.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com