Sentence examples for break up cell from inspiring English sources

The ventral horns were trypsinized in Hank"s solution for 13 minutes, the trypsin solution was replaced with motor neuron (MN) medium with growth factors and agitated to break up cell clumps.

Add 3ml media to the flask and gently pipette to collect all the cells and break up cell clumps.

To break up cell walls and to extract As, a sonic dismembrator (Fisher Scientific, Model 100) operated at power level 2 was employed.

Cell lysates were passed through a 21-gauge needle to break up cell aggregates, and then centrifuged at 14,000 × g for 15 min at 4 °C [ 14].

Fixed cells were pelleted and resuspended in water, then sonicated on a Branson Sonifier Model 450 at 10% amplitude for 3 s to break up cell clumps.

Cells were resuspended in a 50-mL conical tube with addition of 10 mL of HBSS and triturated with a serological pipette to resuspend the cells and break up cell clusters.

Treated samples were pelleted again, resuspended in 0.5 ml 50 mM Tris pH 7.5, and sonicated to break-up cell clumps, if any.

In one experiment, Dr. de Duve noticed that acid phosphatase, an enzyme he had included as a control, was less active than in earlier experiments in which he had used an electric blender instead of a pestle to break up cells.

In this study we present Matrix-Lysis, which breaks up cell membranes and solubilizes the tissue matrix, thereby additionally permitting detection of intracellular bacteria.

Being able to generate intense ultrasonic energy would break up specific cells or cell membranes and inject them with drug particles.

There are 7 lytic transglycosylases which, in cleaving peptidoglycan links could be involved in modellingof the cell or could break up environmental cell wall fragments.