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A one-day break is required if 30 or more pitches are thrown.
A single-strand break is required to initiate repair and to provide strand specificity for human MMR [ 26].
One obligate break is required for a terminal deletion, while two obligate breaks are required for an interstitial deletion.
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In the vast majority of cases (1325 out of 1400), either no loop breaking is required, or the solution discovered after greedily breaking all loops has the same cost as the original solution.
A recent study has also demonstrated that the Mre11/Rad50/NBS1 (MRN) complex, a central component in the cellular response to ionizing radiations and other causes of double-strand breaks, is required for ATR-dependant phosphorylation mechanisms of the protein Smc1 (Structural maintenance of chromosomes 1) [ 44].
Rest breaks are required at designated intervals.
The last four of those were match points in the 12th game, but Schalken held on and that meant a tie-break was required to decide the set.
Frequent breaks are required to prevent fatigue, particularly when reporting complex examinations [23, 24].
After service breaks were again swapped, another tie-break was required, and this time Murray set about it with attacking intent.
A tie-break was required and Kyrgios looked likely to take it when he turned around a 2-0 deficitoto lead 4-3 with a break, but Murray played a magical forehand lob to wrestle back the initiative.
Another tie-break was required and this time it was the Swiss who took charge after crucial double-faults from Berdych at 3-2 and on set point, leaving the Czech player's team - decked out in blue and white stripes, like their man - looking forlorn.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com