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Both overall levels of replication and breadth of tissue tropism may be positively correlated with virulence.
We describe a computational pipeline that uses microarray data to assess the level of expression and breadth of tissue profiles for transcripts with distinct first exons regulated by alternative promoters.
It is probable then, that there are differences in selection on protein sequences of males and females independent of the breadth of tissue expression.
To examine the breadth of tissue expression of genes represented by the top 100 ranked clones, we mined the summary SAGE expression data from the Cancer Gene Anatomy Project http://cgap.nci.nih.gov/.
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By combining diverse types of annotation, we can better understand the function of a given gene in a breadth of tissues and organ systems and of the biological processes it is involved in the organismal level, as well as its role in disease.
The breadth of this tissue and species data allows us to show that downregulation of target genes by miRNAs is more pronounced between tissues than between species and that downregulation is more pronounced for genes with fewer binding sites for expressed miRNAs.
In multicellular organisms the correlation between dN/dS and expression breadth (number of tissues where genes are expressed) is often stronger than with the actual level of gene expression [ 54, 55, 61].
It should be noted that some tissue-specific NTR may not be real, because we could not collect all diverse tissues or sufficient replicates; however the conclusions were based on the comparison of tissue breadth between NTRs and annotated genes in the same background.
A recent study shows that genes' breadth of expression in tissues is positively correlated with the expression level of the genes [ 24].
TE gene fractions and GL were compared with the overall level of gene expression and the breadth of expression across tissues.
This is in line with the results of a previous study [43] showing that different genes belonging to a given biological process (GO category) have a specific breadth of expression (number of tissues where a gene is expressed), suggesting that some cellular functions are more ubiquitous than others.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com