Using blast algorithm, we indirectly evaluated the transcriptome coverage breadth by estimating the number of unique genes.
The average size of biomes did not show phylogenetic signal in any lineage whereas niche breadth, as estimated by number of biomes overlapping with geographic range, displayed phylogenetic signal only for the H. dentipes and Graptodytes lineages.
Gene-wise expression breadth was estimated following Yanai et al. (2005): where N is the number of tissues, x i, the expression intensity for gene x in tissue i, and xmax, the maximum expression intensity of gene x across all tissues.
Finishing steps include identification and removal of overlapping sequences with resulting physical gaps replaced by a variable number of successive "N's", reflecting their estimated breadth.
Table 2 displays GEMS serotypes in relation to the proposed quadrivalent vaccine composition (S. flexneri 2a, S. flexneri 3a, S. flexneri 6 plus S. sonnei) to estimate breadth of coverage and possible variation over time from the perspective of all 7 GEMS sites, the site with the most Shigella cases (Bangladesh), and the other 6 sites.
Therefore, the publication date of the oldest publication, estimating the length of publication history, and the number of publications, estimating the breadth of publication history, were examined for each gene using the Entrez Gene Feb 2007 dataset (Table 2).
Williamson–Hall analysis is a simplified integral breadth method employed for estimating crystal, crystallite size and lattice strain, considering the peak width as a function of 2θ [39].
Williamson–Hall analysis is a simplified integral breadth method employed for estimating crystal, crystallite size and lattice strain, considering the peak width as a function of 2θ (Sundeep et al. 2016).
However, the Williamson–Hall (W H) analysis is a simplified integral breadth method employed for estimating crystallite size and lattice strain, considering the peak width as a function of 2θ [15].
To be conservative in estimating response breadth, responses to 2 overlapping peptides were called one response; to 3 overlapping peptides were called 2 responses.
