Tail vein injections of approximately 15 MBq 18F-FDG in 300 μL of physiological NaCl solution (Braun AG) were followed by rinsing the catheter with 150 μL of physiological NaCl solution.
heparine (ratiopharm, Ulm, Germany) and diluted in "Ringer" isotonic saline solution (Braun, Melsungen, Germany).
Gelofusine (Gelo, Braun) is a solution of succinylated gelatin fragments (40 g/l).
Controls received equal volume of 0.9 % sodium chloride (NaCl) solution (Braun Melsungen AG, Melsungen, Germany).
Animals were perfused with Ringer's solution (Braun Medical AG, Emmenbrücke, Switzerland) into the left cardiac ventricle.
Blood glucose concentration was maintained above 4.5 mmol/l using a continuous infusion of 40% glucose solution (Braun).
Sham animals received a comparable amount of 0.9 % saline solution (Braun, Melsungen, Germany) and served as controls (HAI sham, sCHT sham).
Umbilical cord tissue was washed with povidone-iodine solution (Braun, Melsungen, Germany) and 70% ethanol (apomix, Halle/Salle, Germany) for disinfection.
Continuous infusion of Ringerfundin™ solution (Braun Melsungen Ag, Melsungen, Germany) was used as a fluid replacement in doses of 10 ml kg−1 h−1 during the preparation and reduced to 7 ml kg−1 h−1 thereafter.
The cells were washed again with PBS, and 2 ml of Ringer's solution (Braun) with 10 mM 4- 2-hydroxyethyl -1-piperazineethanesulfonic acid (HEPES) buffer, pH 7.4- 2-hydroxyethyl -1-piperazineethanesulfonic
