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Consequently, a detailed quantification of branching complexity of the dendritic arbor neurons of the dentate nucleus during its development in the prenatal period is lacking.
The neuronal morphology was quantitatively evaluated with four parameters which describe two cell features: neuron size and branching complexity of the dendritic arbor.
In addition to the reduced branching complexity of the oligodendrocyte processes, the overall cell size was reduced and (g)), suggesting that the ability of oligodendrocytes to extend processes is compromised upon culture on ephrin-A1.
Simultaneously using two mathematical analyses (Section 2.2), we calculated four parameters which quantify two key cell features: the size of the neuron field and branching complexity of the dendritic arbor.
Thus, to our knowledge, this is the first time that a mathematical model which quantitatively describes the neuron size and branching complexity of the dendritic arbor in prenatal development is used.
Developmental PCB exposure caused an apparent delay in dendritic growth in hippocampal CA1 pyramidal neurons at weaning as evidenced by a significant decrease in branching complexity of the distal basilar dendritic trees at PND22.
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Morphometric analysis showed that the branching complexity of 11-week-old Lrp6+/− glands was decreased by 17% compared with that of littermate wild-type mice (p = 8.4×10−3) (Fig. 3C).
The increased branching complexity of Sema3f −/−;Sema3g lacZ/lacZ double mutants may be due to an increased tip cell formation (Fig. 3J).
Thus, this study attempts to quantify the morphology of neurons from the dentate nucleus at various gestational periods, analyzing the size of neurons and branching complexity of dendritic arbor.
For example, the translational repressor Nanos (Nos) regulates dendrite morphogenesis and branching complexity of Drosophila class IV da neurons.
Moreover, treatment of organotypic cerebellar cultures with sphingomyelinase was demonstrated to enhance dendritic branching complexity of Purkinje cells (23).
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