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Additionally, bootstrap values were <100% for internal branches within the subcluster I, Figure 3(b).
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The finest consistent term for each subcluster is found by considering all PSI-MI terms on the paths from the subcluster members to its maximum — the search is not restricted to those terms that are within the subcluster.
Finally, the divergence observed for DEHA2F16478g (subcluster C3 and SONS d) may be linked to a species-based clustering of DEHA genes within the subcluster C3.
Within the subcluster comprised of the rose sequences, there is no separation of R. multiflora and R. rugosa sequences.
The number of adjacent cells within the subcluster highly correlates with the Euclidean 3D distance between two DCN soma.
These subclusters were chosen based on natural divisions in phage similarity seen in the dot plot, but could be more strictly defined by ANI values of at least 66% between two phages within the subcluster.
Each consolidated interaction is assigned a single PSI-MI term for interaction-detection method that most specifically describes the entire collection of annotation terms within the subcluster.
Though minor changes were evident within the subclusters of the major varietal types, the relative position of the major clusters remained preserved.
We chose a cutoff to achieve a balance between the size of the biggest subcluster and the average correlation within the biggest subcluster.
Regarding the VP1, NSP2, NSP3, NSP4, and NSP5 gene segments, human rotavirus strains Dhaka6 and Matlab36 02 are localized within the human subcluster, whereas strain KTM368 formed a distinct branch inside genotype 1 and did not cluster with any known human or porcine rotavirus strain (Technical Appendix 2).
Both subclusters were supported by 1,000 bootstrap replications and were consistent with the taxa distance (data not shown) showing introductions of GI in 1982 (within the GIa subcluster followed by a recent dispersion all over the country), and in 1992 for the GIb subcluster followed by local transmission.
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