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The diameter of the trunk and all secondary branches were measured periodically with a pair of callipers, while the lengths of the secondary branches were measured using a graduated pole.
Comparing the maximum height to which branches were measured using TreeD with the PSP mean height data, it was estimated that the branches measured would be 11 years of age or older, and would occur in the lower half of the green crown.
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For trichome size analysis, the height of stalks and branches was measured using Image Tool 3.0 (http://ddsdx.uthscsa.edu/dig/itdesc.html).uthscsa.edu/dig/itdesc.html
The aspect and inclination of each branch was measured using a standard compass with clinometer.
Branch diameters were measured using a photogrammetric technique, TreeD, on pre-selected sample trees from unpruned treatments in two silviculture experiments, one in the North Island and one in the South Island of New Zealand.
The tibial bone gene expressions of RANKL, OPG and MCSF were measured using the branch DNA technique.
Following two months of treatment, the rats were euthanized and the gene expressions of BMP-2, OPG, RANKL and MCSF in the femoral bones were measured using a branch - DNA technique.
Number and length of processes, including axonal and dendritic branches, as well as areas of cell bodies were measured using Image J (National Institutes of Health, Bethesda, MD).
ROS were measured using DCF.
Angles were measured using ImageJ.
dsRNA concentrations were measured using biophotometer (Eppendorf).
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