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Briefly, coral tissue from frozen coral branches was removed using an airbrush and filtered (0.45 µm) seawater solution.

Coral tissue from frozen coral branches was removed using an airbrush and filtered (0.45 µm) seawater solution.

Coral tissue from frozen coral branches was removed using an airbrush and Symbiodinium sp. cells were separated as described above.

Bacterial cells found on the dead coral branch were removed using a sterile scalpel and immediately placed into KESM media and kept under dim visible light for three days.

To avoid any bias and potential tissue lesions due to fragmentation process, for each sample, a small basal portion of the coral branch (1 cm) was removed using wire cutters.

IgG was removed using Protein G Sepharose Beads.

When these cells were removed using trypsin, the wild-type mesenchyme rescued the branching phenotype (Fig. 7C).

The tusks have been removed using chainsaws.

Vitelline membranes were removed using forceps.

Duplicates were removed using Picard.

Rate of wound closure when branches were removed with stem or branch of origin (flush cut pruning) and cuts made away from the branch collar (natural target pruning) were compared on all species over a 3-year period.