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h1 and hIJ3 mutant embryos did not show defects in formation of the primary tracheal branches (data not shown); however, in embryos trans-heterozygous for h22 and h1J3, the DT was broken (data not shown).
The normal structure of thoracic viscera and their branches (data not shown) suggested that altered cardiovascular or pulmonary function a common etiology for perinatal mortality was also not the cause of lethality in this case.
Comparisons of substitution rates between the different lineages were highly non significant at 1.25%, indicating that all species evolve at very similar rates, thus rendering unlikely a possible artefact caused by long branches (data not shown).
Bayesian analysis of the same alignment retained the same topology and supported the same branches (data not shown).
The tree topologies produced by these three algorithms were identical except for the interior branches (data not shown).
Assignment of genes at the subfamily level is identical using either maximum likelihood or neighbor joining methods, although some differences are manifested at deeper branches (data not shown).
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The two species that clustered most closely with Olpidium in the Bayesian analysis, Piptocephalis corymbifera and Rhopalomyces elegans, had long-branches (data not shown), were missing data from two loci, and did not cluster with Olpidium in the likelihood analysis.
In addition to early flowering, HD reduced leaf longevity and increased branching (data not shown).
Macaque RPS4Y2 has evolved at a greater ω than the other branches (0.6147 and 0.0581, respectively), except for human RPS4Y2, which could indicate a relaxation of purifying selection in this branch (data not shown).
From 71 RNA-seq contigs of the barley NRT1/PTR family only 45 cluster into the four subfamilies defined by Tsay et al. [ 14], while 26 sequences form a separate branch (data not shown).
Analogous estimates for the insect-deuterostome comparison yielded extremely small numbers (0 in most of the sampling experiments) owing to the short deuterostome branch (data not shown) compared to the number of RGC_CAMs supporting the coelomate clade.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com