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(b) Giraffe FOLR1 contains seven substitutions that each show evidence of positive selection (P<0.05) by the branch-site model.
The revised branch-site model A was used, which attempts to detect positive selection acting on a few sites on particular specified lineages, that is, 'foreground branches'61.
This approach corresponds to the branch-site model.
Therefore, the branch-site model [25], [26] was applied.
Each branch-site model was run multiple times to ensure convergence of log-likelihood values.
Both the two-ratio and branch-site model fail to detect any evidence for positive selection.
The branch-site model analysis shows that positive selection accelerates Tetherin evolution in ruminant lineage.
To address this issue, the branch-site model implemented in the codeML program in the PAML package was used.
The branch-site model makes rather stringent assumptions about the selective pressures acting on the foreground and background branches.
The branch-site model detected positive selection acting on the ancestral branch of the New World Monkey lineage, suggesting an episodic adaptive evolution.
We use the branch-site model [23], [49] to detect such temporal changes in positive selection pressure, as implemented in the codeml program in the paml package [25].
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