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Pearson's correlation heatmaps (bamCorrelate bins, −-corMethod pearson) and heatmap representations of signal intensity (computeMatrix reference-point followed by heatmapper in the multiheatmapper branch) were generated using deepTools suite [ 28].
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The unrooted phylogenetic tree with unscaled distance branches were generated using the program MEGA3.1 http://meme.sdsc.edu/meme4_1/intro.html with the Neighbor-Joining method.
The unrooted phylogenetic tree with unscaled distance branches was generated using the program MEGA 3.1 http://meme.sdsc.edu/meme4_1/intro.html with the Neighbor-Joining method.
Multiple sequence alignment and dendrogram with branch length were generated using CLUSTAL W Multiple Sequence Alignment Program with default settings (http://www.genome.jp/tools-bin/clustalw).
Majority-ruled parsimonious trees were generated using the program protpars of PHYLIP [ 35] with maximum likelihood branch lengths calculated using TREE-PUZZLE [ 36].
The phylogenetic relationships of fourteen B. cenocepacia strains/isolates, including our six isolates of interest, were generated using the Neighbor-Joining method60 with 100 bootstrap replicates undertaken to display branch supports61.
Graphics were generated using R36.
Molecular images were generated using UCSF Chimera57.
Sashimi plots were generated using MISO (v.0.5.4).
All figures were generated using Pymol [31].
Graphs were generated using WinMDI (Scripps).
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